Our Services
Sanger Sequencing Services
Features of 1st BASE DNA Sequencing Services:
- High quality and long read lengths (~ 1000 bases) for pure DNA template
- Wide range of free sequencing primers available
- Stringent automated processes to include control reaction in each of our processes.
- Only QC passed sequencing reaction(s) released to our valued customer.
- 1st BASE Online ordering (1oo) system allows monitoring your order status on real-time.
Next Generation Sequencing Services (NGS)
NGS is driving growth and possibilities in scientific research as DNA is sequenced at unprecedented speed. It has opened new worlds in genomic research and resulted in novel biological applications in diverse fields of science.
1st BASE offers the latest in NGS technologies, together with cross-platform expertise in choosing the most suitable technology to meet your sequencing needs. Get in touch with us and let us recommend the most suitable or combination of sequencing approach for your project with no obligations.
- Illumina MiSeq, NovaSeq 6000 Sequencing Technology
- Pacific Biosciences Sequel II Sequencing Technology
- Nanopore GridION Sequencing Technology
Molecular Biology Services
1st BASE offer wide range of Molecular Biology Services that allows you to tap on our expertise and thus saves you previous time to focus on other important issues. When we manage your project, all information will be kept in strict confidence.
Every project will also be carefully documented for traceability, accountability and reproducibility.
- Sample Preparation
- PCR
- Cloning
- Genotyping
- DNA Barcoding Services
Fragment Analysis Services
Fragment analysis is used to describe genetic marker analysis experiments which rely on detection of changes in the length of a specific DNA Sequence to indicate the presence of absence of a genetic marker.
In genetic marker analysis, the sequence of the gene is not directly analyzed. Genetic markers are usually polymorphic genetic sequences contained in or near an allele of interest, such as microsatellite or RFLP, which allow the chromosomal alleles to be distinguished.
1st BASE offer DNA Fragment Analysis service where fluorescently fragments are detected using Applied Biosystems Genetic Analyzer and then interpreted using the GeneMapper® v 4.0 analysis software.
Human Cell Line Authentication
As outlined in ASN-0002, the standard short tandem repeat (STR) genotyping uses at least eight STR loci (TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317, and D5S818) plus Amelogenin for gender identification for human cell line authentication.
1st BASE Human Cell Line Authentication Service offers the even higher power of discrimination with the analysis of 24 loci. Twenty-four short tandem repeat (STR) loci plus the gender determining locus, Amelogenin, is amplified using GenePrint® 24 System from Promega. By comparing the STR profile against the database profile, we can make accurate identification of the cell lines.